nfo [2017-06-27 18:05:32]

type IV apurinic/apyrimidinic endonuclease

locus: BSU25130

pI: 5.37

mw: 32.92 kDa

protein length: 297 aa

gene length: 891 bp

function: repair of oxidative DNA damage in spores

product: type IV apurinic/apyrimidinic endonuclease

essential: no

synonyms: yqfS

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.2|A/P endonucleases]

Gene

Coordinates: 2,593,281 → 2,594,174

Phenotypes of a mutant

• an ''[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|exoR] [gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]'' double mutant is impaired in germination and spore outgrowth due to the accumulation of DNA lesions, this can be rescued by inactivation of ''[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]'' [Pubmed|24244006]
• an ''[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|exoR] [gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]'' double mutant is sensitive to radiation [Pubmed|24123749]

The protein

Catalyzed reaction/ biological activity

• Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products (according to Swiss-Prot)

Protein family

• AP endonuclease 2 family (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]-[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C462 (yqfS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2460 NBRP B. subtilis, Japan]
• available in [[Mario Pedraza-Reyes]]' lab
• GP899 (Δ''nfo''::''kan'') and GP1502 (Δ''nfo''::''cat''), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 22933559

Original publications

• 24244006,18203828,16237020,12949090,19930460,12486072,21441501,24123749,24123749,24914186